cell culture human sarcoma cell lines ht1080  (ATCC)

Journal: American Journal of Translational Research

Article Title: A novel method for potentiation of chemotherapy in soft tissue sarcomas with BromAc

doi:

Figure Lengend Snippet: 72-hour treatment with doxorubicin. Top graphs represent (A) HT1080, (B) SW872, (C) VAESBJ, and (D) SW982 cell proliferation compared to an untreated control. Significance is denoted by (*) such that P-values are represented by P<0.05 (*), P<0.005 (**), P<0.0005 (***), and P<0.0001 (****). Bottom graphs represent dose-response curve of (E) HT1080, (F) SW872, (G) VAESBJ, and (H) SW982 cell lines with associated IC50, calculated using Prism GraphPad 7.

Article Snippet: Cell culture Human sarcoma cell lines HT1080, SW872, VAESBJ and SW982 were attained from the American Type Culture Collection (ATCC, USA).

Techniques: Control

Journal: American Journal of Translational Research

Article Title: A novel method for potentiation of chemotherapy in soft tissue sarcomas with BromAc

doi:

Figure Lengend Snippet: Efficacy of 72-hour treatment with doxorubicin alone and in combination with bromelain and acetylcysteine in (A) HT1080, (B) SW872, (C) VAESBJ, and (D) SW982 cells measured through proliferation compared to untreated controls. Data are presented as mean ± SD.

Article Snippet: Cell culture Human sarcoma cell lines HT1080, SW872, VAESBJ and SW982 were attained from the American Type Culture Collection (ATCC, USA).

Techniques:

Journal: American Journal of Translational Research

Article Title: A novel method for potentiation of chemotherapy in soft tissue sarcomas with BromAc

doi:

Figure Lengend Snippet: Combination index of doxorubicin in varying combinations with bromelain and acetylcysteine in (A) HT1080, (B) SW872, (C) VAESBJ, and (D) SW982 cell lines. The dotted lines represent thresholds for Chou-Talalay antagonism (CI>1.1) as well as synergy (CI<0.9). Additive effect corresponds to (0.9

Article Snippet: Cell culture Human sarcoma cell lines HT1080, SW872, VAESBJ and SW982 were attained from the American Type Culture Collection (ATCC, USA).

Techniques:

Journal: American Journal of Translational Research

Article Title: A novel method for potentiation of chemotherapy in soft tissue sarcomas with BromAc

doi:

Figure Lengend Snippet: Combination Index of doxorubicin in varying combinations with bromelain and acetylcysteine, displayed for various concentrations of doxorubicin

Article Snippet: Cell culture Human sarcoma cell lines HT1080, SW872, VAESBJ and SW982 were attained from the American Type Culture Collection (ATCC, USA).

Techniques:

Journal: American Journal of Translational Research

Article Title: A novel method for potentiation of chemotherapy in soft tissue sarcomas with BromAc

doi:

Figure Lengend Snippet: Combination index of doxorubicin in varying combinations with bromelain and acetylcysteine, displayed for various concentrations of doxorubicin

Article Snippet: Cell culture Human sarcoma cell lines HT1080, SW872, VAESBJ and SW982 were attained from the American Type Culture Collection (ATCC, USA).

Techniques:

Journal: American Journal of Translational Research

Article Title: A novel method for potentiation of chemotherapy in soft tissue sarcomas with BromAc

doi:

Figure Lengend Snippet: Mucin depletion as assessed quantitatively through ImageJ RGB analysis

Article Snippet: Cell culture Human sarcoma cell lines HT1080, SW872, VAESBJ and SW982 were attained from the American Type Culture Collection (ATCC, USA).

Techniques: Control

Journal: American Journal of Translational Research

Article Title: A novel method for potentiation of chemotherapy in soft tissue sarcomas with BromAc

doi:

Figure Lengend Snippet: Immunofluorescence of soft tissue sarcoma HT1080 cells following 48 hours of treatment with bromelain, acetylcysteine, and bromelain plus acetylcysteine. Expression of MUC1 was viewed under confocal microscope with red and green fluorescence corresponding to nucleus and MUC1 staining, respectively. Scale bar: 50 μm. Final magnification =600×.

Article Snippet: Cell culture Human sarcoma cell lines HT1080, SW872, VAESBJ and SW982 were attained from the American Type Culture Collection (ATCC, USA).

Techniques: Immunofluorescence, Expressing, Microscopy, Fluorescence, Staining

Journal: American Journal of Translational Research

Article Title: A novel method for potentiation of chemotherapy in soft tissue sarcomas with BromAc

doi:

Figure Lengend Snippet: Immunofluorescence of soft tissue sarcoma HT1080 cells following 48 hours of treatment with bromelain, acetylcysteine, and bromelain plus acetylcysteine. Expression of MUC4 was viewed under confocal microscope with red and green fluorescence corresponding to nucleus and MUC4 staining, respectively. Scale bar: 50 μm. Final magnification =600×.

Article Snippet: Cell culture Human sarcoma cell lines HT1080, SW872, VAESBJ and SW982 were attained from the American Type Culture Collection (ATCC, USA).

Techniques: Immunofluorescence, Expressing, Microscopy, Fluorescence, Staining

Journal: American Journal of Translational Research

Article Title: A novel method for potentiation of chemotherapy in soft tissue sarcomas with BromAc

doi:

Figure Lengend Snippet: Western blot analysis of a panel of biomarkers for apoptosis, cell cycle progression, and autophagy in soft tissue sarcoma HT1080 and SW872 cells.

Article Snippet: Cell culture Human sarcoma cell lines HT1080, SW872, VAESBJ and SW982 were attained from the American Type Culture Collection (ATCC, USA).

Techniques: Western Blot

Journal: American Journal of Translational Research

Article Title: A novel method for potentiation of chemotherapy in soft tissue sarcomas with BromAc

doi:

Figure Lengend Snippet: 72-hour treatment with doxorubicin. Top graphs represent (A) HT1080, (B) SW872, (C) VAESBJ, and (D) SW982 cell proliferation compared to an untreated control. Significance is denoted by (*) such that P-values are represented by P<0.05 (*), P<0.005 (**), P<0.0005 (***), and P<0.0001 (****). Bottom graphs represent dose-response curve of (E) HT1080, (F) SW872, (G) VAESBJ, and (H) SW982 cell lines with associated IC50, calculated using Prism GraphPad 7.

Article Snippet: Human sarcoma cell lines HT1080, SW872, VAESBJ and SW982 were attained from the American Type Culture Collection (ATCC, USA).

Techniques: Control

Journal: American Journal of Translational Research

Article Title: A novel method for potentiation of chemotherapy in soft tissue sarcomas with BromAc

doi:

Figure Lengend Snippet: Efficacy of 72-hour treatment with doxorubicin alone and in combination with bromelain and acetylcysteine in (A) HT1080, (B) SW872, (C) VAESBJ, and (D) SW982 cells measured through proliferation compared to untreated controls. Data are presented as mean ± SD.

Article Snippet: Human sarcoma cell lines HT1080, SW872, VAESBJ and SW982 were attained from the American Type Culture Collection (ATCC, USA).

Techniques:

Journal: American Journal of Translational Research

Article Title: A novel method for potentiation of chemotherapy in soft tissue sarcomas with BromAc

doi:

Figure Lengend Snippet: Combination index of doxorubicin in varying combinations with bromelain and acetylcysteine in (A) HT1080, (B) SW872, (C) VAESBJ, and (D) SW982 cell lines. The dotted lines represent thresholds for Chou-Talalay antagonism (CI>1.1) as well as synergy (CI<0.9). Additive effect corresponds to (0.9

Article Snippet: Human sarcoma cell lines HT1080, SW872, VAESBJ and SW982 were attained from the American Type Culture Collection (ATCC, USA).

Techniques:

Journal: American Journal of Translational Research

Article Title: A novel method for potentiation of chemotherapy in soft tissue sarcomas with BromAc

doi:

Figure Lengend Snippet: Combination Index of doxorubicin in varying combinations with bromelain and acetylcysteine, displayed for various concentrations of doxorubicin

Article Snippet: Human sarcoma cell lines HT1080, SW872, VAESBJ and SW982 were attained from the American Type Culture Collection (ATCC, USA).

Techniques:

Journal: American Journal of Translational Research

Article Title: A novel method for potentiation of chemotherapy in soft tissue sarcomas with BromAc

doi:

Figure Lengend Snippet: Combination index of doxorubicin in varying combinations with bromelain and acetylcysteine, displayed for various concentrations of doxorubicin

Article Snippet: Human sarcoma cell lines HT1080, SW872, VAESBJ and SW982 were attained from the American Type Culture Collection (ATCC, USA).

Techniques:

Journal: American Journal of Translational Research

Article Title: A novel method for potentiation of chemotherapy in soft tissue sarcomas with BromAc

doi:

Figure Lengend Snippet: Mucin depletion as assessed quantitatively through ImageJ RGB analysis

Article Snippet: Human sarcoma cell lines HT1080, SW872, VAESBJ and SW982 were attained from the American Type Culture Collection (ATCC, USA).

Techniques: Control

Journal: American Journal of Translational Research

Article Title: A novel method for potentiation of chemotherapy in soft tissue sarcomas with BromAc

doi:

Figure Lengend Snippet: Immunofluorescence of soft tissue sarcoma HT1080 cells following 48 hours of treatment with bromelain, acetylcysteine, and bromelain plus acetylcysteine. Expression of MUC1 was viewed under confocal microscope with red and green fluorescence corresponding to nucleus and MUC1 staining, respectively. Scale bar: 50 μm. Final magnification =600×.

Article Snippet: Human sarcoma cell lines HT1080, SW872, VAESBJ and SW982 were attained from the American Type Culture Collection (ATCC, USA).

Techniques: Immunofluorescence, Expressing, Microscopy, Fluorescence, Staining

Journal: American Journal of Translational Research

Article Title: A novel method for potentiation of chemotherapy in soft tissue sarcomas with BromAc

doi:

Figure Lengend Snippet: Immunofluorescence of soft tissue sarcoma HT1080 cells following 48 hours of treatment with bromelain, acetylcysteine, and bromelain plus acetylcysteine. Expression of MUC4 was viewed under confocal microscope with red and green fluorescence corresponding to nucleus and MUC4 staining, respectively. Scale bar: 50 μm. Final magnification =600×.

Article Snippet: Human sarcoma cell lines HT1080, SW872, VAESBJ and SW982 were attained from the American Type Culture Collection (ATCC, USA).

Techniques: Immunofluorescence, Expressing, Microscopy, Fluorescence, Staining

Journal: American Journal of Translational Research

Article Title: A novel method for potentiation of chemotherapy in soft tissue sarcomas with BromAc

doi:

Figure Lengend Snippet: Western blot analysis of a panel of biomarkers for apoptosis, cell cycle progression, and autophagy in soft tissue sarcoma HT1080 and SW872 cells.

Article Snippet: Human sarcoma cell lines HT1080, SW872, VAESBJ and SW982 were attained from the American Type Culture Collection (ATCC, USA).

Techniques: Western Blot

Journal: Redox Biology

Article Title: Ferroptosis-dependent extracellular vesicles from macrophage contribute to asbestos-induced mesothelial carcinogenesis through loading ferritin

doi: 10.1016/j.redox.2021.102174

Figure Lengend Snippet: CD63-positive ferroptosis-dependent EVs (FedEVs) derived from macrophages simultaneously exposed to asbestos and iron in vivo and in vitro . (a) Histological images of peritoneal wall in situ in the murine model 6 months after ip injection of 3 mg crocidolite; hematoxylin and eosin (HE) image (left upper), Berlin blue staining (insoluble iron as bluish green, left lower), phase-contrast with red fluorescent image (right panels); cell-specific marker: MSLN (mesothelin), mesothelial cell; αSMA (smooth muscle actin), myofibroblast; CD68, macrophage; white arrowheads, crocidolite (bar = 100 μm). (b) High-resolution fluorescent image in granuloma; red, cell specific marker; green, CD63; blue, Hoechst33342 nuclear staining (bar = 20 μm). (c) Quantification of fluorescent intensity of CD63 in different cells surrounding granuloma (n = 30; mean ± SEM). (d) Schematic image of peritoneal wall surrounding the asbestos-induced granuloma. (e, g) Immunoblot analysis of CD63 in cells under ferroptosis and their media; HT1080 fibrosarcoma cells were exposed to RSL3 (10 μM, 24 h), and phorbol myristate acetate (PMA)-primed THP1 macrophage cells were exposed to crocidolite (15 μg/cm 2 ) in the presence of absence of ferric ammonium citrate (Fe, 100 μg/ml) up to 96 h. (f, h) Membrane-catch transmission electronmicroscopy (TEM) images of EVs on carbon grids (bar = 200 nm). (i–l) The precision of nanoparticle tracking analysis (NTA) concentration measurements counted size-distribution of EVs derived from HT1080 (24 h) and THP1 (96 h) in different conditions in i and j. NTA concentration measurements counted number of EVs in k and l (mean ± SEM). Refer to text for details. (For interpretation of the references to color in this figure legend, the reader is referred to the Web version of this article.)

Article Snippet: MeT-5A human mesothelial cell line (ATCC), THP1 human macrophage cell line and HEK293T cell line (RIKEN Cell Bank) and HT1080 human sarcoma cell line (JCRB cell bank) were grown under standard sterile cell culture conditions (37 °C, humidified atmosphere, 5% CO 2 ) in EMEM containing 10% FBS and 1% antibiotic-antimycotic (Gibco).

Techniques: Derivative Assay, In Vivo, In Vitro, In Situ, Injection, Staining, Marker, Western Blot, Membrane, Transmission Assay, Concentration Assay

Journal: Redox Biology

Article Title: Ferroptosis-dependent extracellular vesicles from macrophage contribute to asbestos-induced mesothelial carcinogenesis through loading ferritin

doi: 10.1016/j.redox.2021.102174

Figure Lengend Snippet: Mesothelial cells phagocytose FedEVs derived from asbestos-exposed macrophages under ferroptosis. (a) Schema of culture system for the uptake by MeT-5A (recipient) cells of FedEVs from THP1 cells stably expressing GFP-CD63. (b) Fluorescent cellular images of recipient mesothelial cells phagocytosing GFP-positive FedEVs derived from THP1 macrophage cells exposed to asbestos; red, endogenous CD63; bar = 10 μm. (c) Time-course of quantitative colocalization ratio between GFP-CD63 and endogenous CD63 (mean ± SEM). (d, e) FACS analysis of MeT-5A mesothelial cells for catalytic Fe(II) (RhoNox-4, red) after exposure to GFP-CD63-labeled FedEVs derived from HT1080 by ferroptosis-stimulation with RSL3; mean ± SEM). (f, g) FACS analysis of MeT-5A mesothelial cells for catalytic Fe(II) (RhoNox-4, red) after exposure to GFP-CD63-labeled FedEVs derived from THP1 cells by ferroptosis-stimulation with [crocidolite + Fe]; mean ± SEM). (For interpretation of the references to color in this figure legend, the reader is referred to the Web version of this article.)

Article Snippet: MeT-5A human mesothelial cell line (ATCC), THP1 human macrophage cell line and HEK293T cell line (RIKEN Cell Bank) and HT1080 human sarcoma cell line (JCRB cell bank) were grown under standard sterile cell culture conditions (37 °C, humidified atmosphere, 5% CO 2 ) in EMEM containing 10% FBS and 1% antibiotic-antimycotic (Gibco).

Techniques: Derivative Assay, Stable Transfection, Expressing, Labeling